In vivo protective effect of lipopolysaccharide against Pseudomonas aeruginosa exotoxin A in mice

Protective Properties of Nontoxic Recombinant Exotoxin A (Domain I-II) Against Pseudomonas aeruginosa Infection

Background: Antibiotic resistance and the need for long-term treatments especially for chronic infections necessitate the development <span style="fon...

Use of immunogenic moiety of Pseudomonas aeruginosa exotoxin A as a DNA vaccine in experimentally contaminated mice

Background: DNA immunization is an appropriate method to produce an immunological response. Pseudomonas aeruginosa produces exotoxin A which is highly cytotoxic for eukaryotic cells. Since domains II (translocation domain) and 1b of the toxin have antigenic qualities, so they could be  useful candidates to protect against pseudomonas infections. Objectives: To evaluate if recombinant plasmid co...

Pseudomonas aeruginosa exotoxin A and keratitis in mice.

PURPOSE To determine the importance of Pseudomonas aeruginosa exotoxin A (ETA) as a virulence factor in corneal disease. METHODS Isogenic mutants deficient in ETA were constructed in P. aeruginosa strains PAO1 and ATCC 19660 by allelic exchange and then evaluated for virulence in a mouse model of bacterial keratitis. The effect of ETA on adherence to scarified corneal epithelium was assessed ...

Pseudomonas aeruginosa exotoxin: effect on cell cultures.

The Immunomodulatory Effect of Recombinant Exotoxin A of Pseudomonas Aeruginosa on Dendritic Cells Extracted from Mice Spleen

 Background & Objective: Dendritic cell (DC) is as a key cell in activation of immune response against microbes and disease. Therefore, the effect of recombinant exotoxin A of Pseudomonas aeruginosa on the maturity and the activation of DCs was evaluated in this study. Materials & Methods: Recombinant exotoxin A was produced from Pseudomonas aeruginosa DNA. MTT assay was used to evaluate ...